Composite

Part:BBa_K2197510:Design

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-10-13)


Expression of GLUT9 under the control of a constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 691
    Illegal BamHI site found at 1159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
    Illegal NgoMIV site found at 1460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1259
    Illegal BsaI.rc site found at 1620


Design Notes

The Glut 9b is expected to serve the same function as the Ygfu, which is to absorb uric acid into the cell. This is not going to be tested in the assay but rather to use as an demonstration of this idea Incorporated with a human gene(eukaryotic cell) instead of e coli (prokaryotic cells)



Source

homo sapiens

References